This press release features multimedia. View the full release here: https://www.businesswire.com/news/home/20201209005326/en/
Figure A: SARS Co-V2 is visualized in infected lung tissue from 6 different tissue samples with ACD RNAscope probe (pink), and corresponding virus-positive and virus-negative ROIs were selected from a serial section for GeoMx profiling with the Cancer Transcriptome Atlas. (Graphic: Business Wire)
COVID-19 is still poorly understood within the scientific community, and outcomes vary greatly across individual patients. Research efforts have been challenged by the scarcity of patient-derived tissue samples, as well as the necessity of fixing such samples prior to analysis to reduce the risk of viral transmission. However, the ability of GeoMx DSP to perform high-plex, spatial profiling of formalin-fixed paraffin-embedded (FFPE) samples enables researchers to gather more information from each precious sample and rapidly evaluate the body’s response to infection.
“We now have the ability to better understand the diversity of SARS-CoV-2 infections in patient lung samples using the unprecedented spatial transcriptomic and proteomic analysis that the GeoMx Digital Spatial Profiler provides,” said
In this study, FFPE autopsy samples from patients with SARS-CoV-2 infection were analyzed with the GeoMx DSP, using the >1,800+ plex GeoMx COVID-19 Immune Response Atlas and a 79-plex GeoMx protein assay. GeoMx DSP revealed high intra- and inter-patient heterogeneity in the abundance and localization of immune markers. A critical step prior to quantitative profiling with GeoMX DSP is to determine the cells that contain the SAR-CoV-2 virus. Those cells are grouped into regions of interest with high and low viral loads using RNAscope SARs-CoV-2 probes from
“This study detected differences in immunoregulatory markers that were not identified using traditional immunohistochemical staining and whole slide analysis, illustrating the importance of high-plex spatial analysis,” said
Data from this study has been uploaded to NCBI GEO and is accessible to the public for continued analysis. To learn more about the ways that NanoString’s products enable COVID-19 research, and to access the GeoMx images and data from this study, please visit www.nanostring.com/COVID19.
To learn more about NanoString’s GeoMx Digital Spatial Profiler, please visit https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp.
For more information, please visit www.nanostring.com.
Vice President, Investor Relations & Corporate Communications