The importance of the immune response in AD has come to light through recent work in the field. As neuropathological changes, such as the deposition of beta-Amyloid plaques, accumulate, the brain’s innate immune system is activated. A group of researchers lead by
GeoMx DSP was used to profile more than 40 proteins simultaneously in radial maps of the microenvironment surrounding beta-Amyloid-plaques in the brains of human AD patients, showing substantial differences between patients who carry AD-associated risk variants in the gene TREM2, and those who did not. In addition, the authors found that cell-type profiling using the nCounter Neuropathology and Neuroinflammation gene expression panels correlated well with conventional analyses, “demonstrating that the
“The combination of conventional analysis tools with gene expression analysis and spatially resolved multiplexed protein analysis is a game changer for the analysis of human FFPE tissue specimens,” said Dr. Prokop. “Our data suggest that microglia respond to neuronal injury inflicted by pathological protein aggregates, rather than the aggregated protein itself.”
“This study highlights the value of GeoMx Digital Spatial Profiling and nCounter gene expression panels to yield insights in neuroscience, which may have implications for interventional strategies in neurodegenerative diseases.” said
The data for this publication was generated as part of a collaboration with
The GeoMx Digital Spatial Profiler enables researchers to rapidly and quantitatively characterize tissue morphology with a high-throughput, high-plex RNA and protein profiling system that preserves samples for future analyses.
Interested parties can learn more about DSP by visiting https://www.nanostring.com/scientific-content/technology-overview/digital-spatial-profiling-technology.
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Source: NanoString Technologies, Inc.