“We are delighted that our emerging products are garnering so much visibility at AGBT, the bellwether conference for showcasing biological research tools and emerging applications,” said
GeoMx Digital Spatial Profiling
GeoMx DSP enables high throughput multiplex spatial profiling of RNA and protein targets in a variety of sample types, including FFPE tissue sections. The full launch of the GeoMx DSP platform is scheduled to take place at the upcoming meeting of the
- Largest multi-analyte GeoMx DSP study to date: Researchers from
Stanford Universitystudied 150+ FFPE tissue biopsies from breast cancer patients undergoing sequential treatments of HER2 inhibitors and chemotherapy, profiling 40+ proteins and 100+ mRNAs.
- High-Plex RNA profiling with NGS readout: Three independent studies led by leading academic researchers demonstrate GeoMx DSP’s ultra-high-plex RNA chemistry read out using next generation sequencing. Each experiment spatially profiled 1,400+ mRNAs, illustrating the platform’s high precision and opportunity to map biological pathways in space.
- First Results from Spatial Genomics Center of Excellence: Researchers from the
Broad Institutewill share high-plex spatial RNA and protein analysis generated using a beta instrument run on-site covering six different tissue types.
Hyb & Seq Technology
NanoString’s proprietary Hyb & Seq chemistry is designed to enable a workflow that is simpler and faster than current sequencing methods due to the absence of library preparation, enzymes and amplification. Hyb & Seq’s simple workflow, compatibility with a variety of sample types, and ability to sequence both DNA and RNA in parallel offer the potential for an ideal sample-to-answer solution for clinical sequencing. The Hyb & Seq platform is expected to be commercially launched in 2021. At AGBT 2019, studies performed in collaboration with two leading academic researchers demonstrate ongoing progress in technology development:
First Datafrom Fully-Scaled Sequencing Chemistry: Researchers from the University of Cambridgewill present the first sample-to-answer workflow using all 4,096 Hyb & Seq barcodes, sequencing the entire coding region of the TP53 gene.
- Infectious Disease Testing Capability: Researchers from the
Broad Institutewill present a technique for Hyb & Seq-enabled culture-independent pathogen identification and rapid phenotypic antibiotic susceptibility testing.
- Updated Sequencing and Analysis Pipeline:
NanoStringscientists will introduce a novel end-to-end sequencing analysis pipeline, HexSembler™, designed to assemble Hyb & Seq readout and accurately detect genomic variants.
A detailed list of presentations and posters covering the GeoMx DSP and Hyb & Seq technology is provided below. The GeoMx DSP and Hyb & Seq technology are currently for research use only and are not for use in diagnostic procedures.
“GeoMx™ Digital Spatial Profiler: Delivering on the promise of Spatial Genomics”
“Mapping the microenvironment composition in metastatic prostate cancer by multi-analyte profiling using the GeoMx Digital Spatial Profiler”
“Spatially resolving RNA biomarkers using GeoMx Digital Spatial Profiler for early diagnosis and prognosis in melanoma”
GeoMx DSP Oral Presentations:
Spatial and temporal profiling of protein and RNA in the tumor-immune microenvironment during short-term targeted therapy in HER2-positive breast cancer using GeoMx™ Digital Spatial Profiler
Largest multianalyte biomarker discovery study performed to-date on the GeoMx DSP platform. Profiled 40+ proteins and 100+ mRNAs across multiple regions of interest from 150+ FFPE tissue biopsies from breast cancer patients undergoing sequential treatments of HER2 inhibitors and chemotherapy.
Cell Atlases as road maps to human disease
First demonstration of high-plex spatial RNA and protein analysis of 6 different tissue types using a beta instrument at Nanostring’s Spatial Genomics Center of Excellence
Reconstruction of the tumor landscape
Poster #: 119
Study focused on evaluating precision of spatial analysis in preclinical samples as well as performing spatial pathway mapping across multiple tumor samples.
GeoMx DSP Poster:
Spatially resolving RNA biomarkers using GeoMx™ Digital Spatial Profiler for early diagnosis and prognosis of melanoma
Poster #: 113
Demonstrates high level of technical and biological reproducibility of the high-plex RNA chemistry with next generation sequencing readout. Moreover the study focused on identifying biomarkers that predict the likelihood of progression from dysplastic nevi to advance stages of melanoma.
Mapping the microenvironment composition in metastatic prostate cancer by multi-analyte profiling using GeoMx™ Digital Spatial Profiler
Poster #: 115
This study highlights the importance of high-plex spatial profiling to subtype prostate cancers at a resolution that cannot be readily achieved using traditional IHC technology or RNAseq. Using spatial profiling of RNA and protein, unique marker populations associated with typical adenocarcinoma phenotype vs neuroendocrine phenotype can be identified.
Single-cell protein profiling of immune cells within the tumor microenvironment using NanoString GeoMx™ Digital Spatial Profiling
Poster #: 601
This study highlights the resolution of the platform to select, illuminate and spatially profile proteins at single cell scale. This proof of concept experiment demonstrates resolution capability that enables cell phenotyping applications.
In situ RNA expression profiling of 1600+ immuno-oncology targets in FFPE tissue using NanoString GeoMx™ Digital Spatial Profiler
Poster #: 514
This study demonstrates the performance of the high plex spatial RNA chemistry. A 1412 spatial RNA plex panel compatible with next generation sequencing readout was designed to capture tumor and immune biology. The high plex RNA probes were tested across 23 well characterized fixed cell lines as well as FFPE tissue sections to ensure specificity and precision of the assay.
Comprehensive spatial RNA profiling of tumor, microenvironment and immune response pathways with the NanoString GeoMx™ Digital Spatial Profiler
Poster #: 614
High plex spatial RNA profiling of colecteral cancer samples showed differential activation of signaling pathways was influenced by microsatellite instable (MSI) or microsatellite stable (MSS) status but also regional differences within the tissue. Moreover, this study highlights the ability of the high plex spatial RNA chesmitry to profile lowly abundant targets like chemokines.
Hyb & Seq posters:
Simultaneous DNA copy number and digital RNA expression analysis for subtyping of high-grade serous ovarian cancer FFPE samples using the Hyb & Seq™ NGS platform
Poster #: 118
This study demonstrates a complete sample-to-answer Hyb & Seq NGS workflow, comprising of fully developed universal sequencing chemistry, a manufactured instrument and integrated data analysis pipeline, and its ability to sequence entire coding region of TP53 gene and measure copy number alteration and gene expression of relevant genes from clinical FFPE samples.
A Quantitative, Multiplexed RNA Detection Platform for Rapid Pathogen Identification and Phenotypic Antibiotic Susceptibility Testing (AST) using Hyb & Seq™ NGS Platform
Poster #: 908
This study highlights Hyb & Seq enabled culture-independent pathogen identification and rapid phenotypic antibiotic susceptibility testing, saving ~2 days compared to standard of care.
Hyb & Seq™ NGS Platform Sequencing and Analysis Pipeline: Accurate Variant Calling of Single Molecule Targets for SNV and Indel Detection
Authors: Summer Elasady et al.
Poster #: 213
Introduce a novel end-to-end sequencing analysis pipeline, HexSembler, designed to assemble Hyb & Seq readout and detect genomic variants with accuracy
About NanoString Technologies, Inc.
For more information, please visit www.nanostring.com.
Source: NanoString Technologies, Inc.